SimpleBLI

Longitudinal BLI figure builder

About SimpleBLI and file compatibility

Simple bioluminescence analysis for IVIS data

SimpleBLI is a browser-based tool for longitudinal bioluminescence imaging workflows. Import supported IVIS acquisition folders, preserve calibrated radiance values, crop individual animals, normalize every time point to one scale, measure regions of interest, and export figures, plots, and CSV data.

Built for clear, reproducible BLI figures

  • Compare control and treatment groups across time points.
  • Apply one shared radiance scale and color bar to every panel.
  • Plot total flux, mean radiance, or maximum radiance over time.
  • Build Kaplan–Meier survival plots from animal death dates.
  • Export 300 dpi PNG figures, PNG/SVG plots with group legends, and CSV data.

File compatibility

Does SimpleBLI replace acquisition software?

No. SimpleBLI analyses and assembles figures; it does not acquire images or control an instrument. It reads acquisition folders containing ClickInfo.txt and raw luminescent.TIF. Project files with .amix or .aurax extensions are not opened; import the acquisition folder instead.

Can I use ordinary image exports?

Yes. TIFF, PNG, and JPEG images can be arranged as rendered-only panels. Quantitative rescaling requires the original calibration metadata and raw counts.

How is total flux calculated?

Total flux is SimpleBLI’s documented estimate in photons per second, integrated from calibrated radiance across the measured region using a 1 sr approximation. Use it as a consistent measurement within one study. Values from other software are not guaranteed to match.

Is SimpleBLI free?

Yes. SimpleBLI’s analysis and export tools are free, with no installation required. Paid plans increase the number of active cloud experiments: Free keeps 2, Plus keeps 10, and Unlimited has no experiment-count limit. Each cloud backup can be up to 1 GB.